When the wearer has urine, the urine reaches the position of the internal biosensor through the surface of pull-ups.training pants.toddler pants; Under the action of the detection device, the disease markers in urine react with various enzymes fixed on the sensor electrode, and electron transfer occurs to form a weak electrical signal; Next, detect the electrical signal of the device. The analog small signal is converted into digital signal through analog-to-digital converter, and the processor calculates the corresponding glucose or uric acid concentration. The detection results can be transmitted to the smart phone application through the wireless communication module to realize the display and local storage of smart terminal data. In addition, the smartphone application can also upload the test results to the server to realize the long-term storage and online viewing of test data. This is the main process of the intelligent pull-ups system, and then it's the turn to detect.

Intelligent pull-upselectrochemical detection method

Electrochemical detection methods are widely used to detect the concentration of the target or related electrochemical characteristics because of their simple operation, low cost, fast detection speed and high sensitivity. The system needs to control the cost and wearability. Electrochemical detection methods have been widely used in wearable research such as medical detection, environmental monitoring and food safety.
The common electrochemical detection methods are chronoamperometry and cyclic voltammetry. During this period, the voltage applied to the corresponding electrode will promote the fixed enzyme or other electroactive substances on the electrode surface and the target redox reaction. The system uses this method to measure the concentration of glucose or uric acid. The cyclic voltammetry law shows that the voltage increases or decreases uniformly with time. The initial voltage is VO at Ti time point, increases to VL according to a certain growth rate, and then decreases to VO at the same speed. Its increase and decrease time are the same, and it acts repeatedly on the electrode as a triangular wave voltage. This method is often used to detect the concentration of target substance, determine the redox potential of electrode and analyze the reaction properties of electrode.

Principle of intelligent pull-upsglucose and uric acid detection

Based on the electrochemical test methods of the above three electrode systems, the detection principle of glucose and uric acid was discussed. Since glucose oxidase was first used to detect blood glucose, enzyme sensor has become the research focus of scholars for half a century, and has attracted extensive attention in various fields. The method has the advantages of fast detection speed, high specificity and simple manufacture. This paper adopts the second generation enzyme biosensor, which is characterized by analyzing the resulting weak current signal through a professional signal collector, adding the electronic medium and the electron transfer speed between them, so as to realize the electron transfer between the enzyme active center and the working electrode. Because glucose oxidase has high specificity for glucose, this enzyme was selected to detect glucose in urine.
Glucose produces glucolactone and reduced glucose oxidase under the action of glucose oxidase and external excitation voltage. At the same time, the electron mediator will be oxidized and release electrons. The number of electrons is related to the concentration of glucose. The higher the concentration, the more electrons will be produced. The detection device in the system realizes the chronoamperometry, and the current generated by the reaction is calculated as the glucose concentration.

This paper expounds the three electrode system and common detection methods used in electrochemical detection, and introduces the electrochemical detection principle of glucose and uric acid, which lays a theoretical foundation for the detailed design of subsequent sensors, system hardware circuits and software. It also provides a reference for the implementation of intelligent pull-ups, training pants and toddler pants.